Gary Cass has been running a few workshops for SymbioticA residents. On Monday we did an electrophoresis gel workshop. We took a plasmid and cut it with restriction enzymes and then ran the DNA on a gel to work out how long the fragments were. If you do this with a few different restriction enzymes it is possible to tell where the plasmid is being cut by each enzyme by comparing the different lengths of the fragments. Gary got us to try and calculate this but none of us really managed to do it although I felt like I was getting close before we ran out of time. I took a few pics of the lab.
Here we have Marie-Pier Boucher, a Candian resident, putting the DNA in the gel. This is quite difficult because you have to be really precise.
And here is a picture of the gel after we let in run for 30mins. The ethidium bromide (highly toxic substance) used to mark the DNA fluoresces under UV. However, the bright pinkish red that you can see is not DNA but RNA. The DNA is dark blue-black.